Biotechnology Online for freshers by AMI BioScience

CSCCG offered 7 day’s hands-on training in Molecular Cloning and western Blotting techniques

Course module, individual hands-on training in Molecular Cloning and Western Blotting, (optional) Genomics and miRNA Genomics tools

1) Molecular Cloning
Gene sequence analysis
Gene and protein sequence blast
Pairwise sequence alignment, Multiple sequence alignment
Designing of PCR primers
Plasmid Plasmid DNA Isolation, and agarose gel confirmation
Restriction Digestion
Digested DNA Extraction From Agarose Gel
Ligation
Competent Cells
Plasmid transformation
Clone confirmation by colony PCR
2) Western Blotting techniques
Protein sample isolation
Protein quantification
SDS-PAGE
Wet-blot and semi dry membrane transfer
Primery antibody and secondary antibody hybridization
Membrane development
Membrane striping and re-hybridization with primery and secondary antibodies

we also offered summer project, summer internship training, Independent research Project for who completed B.Tech/M.tech/ M.Sc. students in the area of Biology, Biotechnology and BioMedical science